Question

Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.

A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.

Explain the basis on which gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilised.

The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow :