Question

Describe the approach of gene therapy used for the treatment of a 4-year-old girl suffering from Adenosine Deaminase Deficiency.

Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.

A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.

Explain the basis on which gel electrophoresis technique works. Write any two ways the products obtained through this technique can be utilised.