Question

The basic scheme of the essential steps involved in the process of recombinant DNA technology is summarised below in the form of a flow diagram. Study the given flow diagram and answer the questions that follow. Step 1 Vector DNA (Plasmid) + Alien DNA (cut using Restriction Enzyme) (cut using Restriction Enzyme) Step 2 Recombinant DNA molecule Step 3 Transfer of recombinant DNA molecule in E. coli (Host) Step 4 Replication of the recombinant DNA molecule in E. coli to form multiple copies of the alien gene

(A) Differentiate between Recombinant DNA and cDNA. (B) Explain the method to increase the competency of the bacterial cell membrane to take up recombinant DNA.