Put one drop of dilute Hydrochloric acid (HCl) on the root tips to soften the tissue. Rinse the roots after 10–15 minutes. Then add 2–3 drops of aceto-carmine stain on them.

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Cell Division by Mitosis | PadhAiPro

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TextCell Division by Mitosis

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Take a jar and fill it up with plain water.

TextCell Division by Mitosis

Now, place an onion bulb over the jar in such a way that its base bearing roots, is immersed in the water.

TextCell Division by Mitosis

Leave the setup for 5–6 days and observe. Do you observe the roots growing? Cut 2–3 cm of the freshly grown roots and transfer them to freshly prepared aceto-alcohol (glacial acetic acid:ethanol :: 1:3). Keep the root tips in aceto-alcohol for 24 hours and then transfer them to 70 per cent ethanol (for preservation).

TextCell Division by Mitosis

Take one or two preserved roots, wash them in water and then, place them on a clean slide.

TextCell Division by Mitosis

Leave the slide for 5–10 minutes and then, gently warm it (with caution) over a spirit lamp.

TextCell Division by Mitosis

Cut the tip portion of the root on the slide and put a coverslip. Gently squash the coverslip with your thumb to spread the cells on the slide.

TextCell Division by Mitosis