Question

(a) Explain the importance of 'ori' and 'rop' in the E. coli cloning vector shown in the above given diagram. (b) Differentiate between exonucleases and endonucleases.

(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.

Assertion (A) : Restriction endonuclease recognises palindromic sequence in DNA and cuts them. Reason (R) : Palindromic sequence has two unique recognition sites PstI and PvuI recognised by restriction endonuclease.

(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.