Assertion (A) : Restriction endonuclease recognises palindromic sequence in DNA and cuts them. Reason (R) : Palindromic sequence has two unique recognition sites PstI and PvuI recognised by restriction endonuclease.

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Assertion-ReasonRestriction Enzymes in BiotechnologyCBSE PYQ

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Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3'

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(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.

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(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.

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