Question

Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.

A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.

Describe in brief any two techniques that can be utilised to transfer recombinant DNA into the host cell directly without using any vector.

Assertion (A): A patient of ADA-deficiency requires periodic or repeated infusion of genetically-engineered lymphocytes. Reason (R): Lymphocytes are not immortal, but have life span.