Observe the given sequence of nitrogenous bases on a DNA fragment and answer the following questions:

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CASE_STUDIESRestriction Enzymes in BiotechnologyCBSE PYQ3 sub-questions

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Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3'

TextRestriction Enzymes in Biotechnology

Name the restriction endonuclease that recognizes this sequence.

TextRestriction Enzymes in Biotechnology

How are sticky ends produced ? Mention their role.

TextRestriction Enzymes in Biotechnology

(a) Explain the importance of 'ori' and 'rop' in the E. coli cloning vector shown in the above given diagram. (b) Differentiate between exonucleases and endonucleases.

CASE_STUDIESRestriction Enzymes in BiotechnologyCloning Vectors in Genetic Engineering

(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.

CASE_STUDIESRestriction Enzymes in Biotechnology

Assertion (A) : Restriction endonuclease recognises palindromic sequence in DNA and cuts them. Reason (R) : Palindromic sequence has two unique recognition sites PstI and PvuI recognised by restriction endonuclease.

Assertion-ReasonRestriction Enzymes in Biotechnology