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Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3'
Name the restriction endonuclease that recognizes this sequence.
How are sticky ends produced ? Mention their role.
Assertion (A) : Restriction endonuclease recognises palindromic sequence in DNA and cuts them. Reason (R) : Palindromic sequence has two unique recognition sites PstI and PvuI recognised by restriction endonuclease.
(a) Write the palindromic nucleotide sequence for following sequence of DNA segment : 5' – GAATTC – 3' (b) Name the restriction endonuclease that recognizes this sequence. (c) How are sticky ends produced ? Mention their role.
Derive a name for a restriction endonuclease which was extracted in the 5th order from the RY13 strain of E. coli.
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