Question

Describe the technique of a typical agarose gel electrophoresis used for the separation and isolation of DNA fragments.

A patient with ADA deficiency requires periodic infusion of genetically engineered lymphocytes. Explain why such periodic infusion is required and also suggest a permanent cure for such ADA deficiency.

In humans, somatic gene therapy was carried out to correct an immunodeficiency disease. Name this disease.

State the steps involved in the process of gene therapy for the treatment of ADA – deficiency.